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AFLP Analysis of Trichoderma spp. from India Compared with Sequence and Morphological-based Diagnostics

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Relation http://oar.icrisat.org/2972/
http://dx.doi.org/10.1111/j.1439-0434.2005.00989.x
 
Title AFLP Analysis of Trichoderma spp. from India Compared with Sequence and Morphological-based Diagnostics
 
Creator Buhariwalla, H K
Srilakshmi, P
Kannan, S
Kanchi, R S
Chndra, S
Satyaprasad, K
Waliyar, F
Thakur, R P
Crouch, J H
 
Subject Groundnut
 
Description Trichoderma species offer considerable potential for controlling aflatoxin contamination in groundnut and other crops. Initial classification of 48 Trichoderma isolates, derived from four different groundnut cultivation sites in India was based on alignment of 28S rDNA sequences to GenBank sequences of ex-type strains. This was found to be substantially more reliable than our routine morphological characterization, but did not provide a comprehensive diagnostic solution, as unique single nucleotide polymorphism (SNP) haplotypes could not be identified for all species. However, all the Trichoderma isolates could be readily distinguished by amplified fragment length polymorphism (AFLP) analysis, based on six primer pair combinations, which generated 234 polymorphic bands. In addition, individual AFLP bands were identified which differentiate closely related species. Similarly, AFLP bands were identified that correlated with different types of antagonism to Aspergillus flavus. The implications of these results for the development of simple polymerase chain reaction (PCR)-based diagnostic assays for antagonistic isolates of Trichoderma is discussed.
 
Publisher Blackwell Publishing
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/2972/1/AFLP_analysis_of_Trichoderma_spp._from_India_compared_with_sequence_and_morphological-based_diagnostics.pdf
Buhariwalla, H K and Srilakshmi, P and Kannan, S and Kanchi, R S and Chndra, S and Satyaprasad, K and Waliyar, F and Thakur, R P and Crouch, J H (2005) AFLP Analysis of Trichoderma spp. from India Compared with Sequence and Morphological-based Diagnostics. Journal Of Phytopathology, 153 (7-8). pp. 389-400. ISSN 1439-0434