Development and Application of an Indirect Competitive Enzyme-Linked Immunoassay for Aflatoxin M1 in Milk and Milk-Based Confectionery
OAR@ICRISAT
View Archive InfoField | Value | |
Relation |
http://oar.icrisat.org/2984/
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Title |
Development and Application of an Indirect Competitive Enzyme-Linked Immunoassay for Aflatoxin M1 in Milk and Milk-Based Confectionery |
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Creator |
Devi, K T
Mayo, M A Hall, A J Craufurd, P Q Wheeler, T R Waliyar, F Subramanyam, A Reddy, D V R |
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Subject |
Groundnut
Food and Nutrition |
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Description |
High-titer rabbit polyclonal antibodies to aflatoxin M1 (AFM1) were produced by utilizing AFM1- bovine serum albumin (BSA) conjugate as an immunogen. An indirect competitive enzyme-linked immunosorbent assay was standardized for estimating AFM1 in milk and milk products. To avoid the influence of interfering substances present in the milk samples, it was necessary to prepare AFM1 standards in methanol extracts of certified reference material (CRM) not containing detectable AFM1 ( |
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Publisher |
American Chemical Society
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Date |
2000
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Language |
en
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Rights |
—
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Identifier |
http://oar.icrisat.org/2984/1/Development_and_application_of_an_indirect_competitive_enzyme-linked_immunoassay_for_aflatoxin_M-1_in_milk_and_milk-based.pdf
Devi, K T and Mayo, M A and Hall, A J and Craufurd, P Q and Wheeler, T R and Waliyar, F and Subramanyam, A and Reddy, D V R (2000) Development and Application of an Indirect Competitive Enzyme-Linked Immunoassay for Aflatoxin M1 in Milk and Milk-Based Confectionery. Journal of Agriculture and Food Chemistry, 50. pp. 933-937. ISSN 0021-8561 |
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