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A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues

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Relation http://oar.icrisat.org/3001/
http://dx.doi.org/10.1007/s00122-006-0448-2
 
Title A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues
 
Creator Marcel, T C
Varshney, R K
Barbieri, M
Jafary, H
Kock, M J D de
Graner, A
Niks, R E
 
Subject Genetics and Genomics
 
Description A consensus map of barley was constructed
based on three reference doubled haploid (DH) populations
and three recombinant inbred line (RIL) populations.
Several sets of microsatellites were used as
bridge markers in the integration of those populations
previously genotyped with RFLP or with AFLP markers.
Another set of 61 genic microsatellites was
mapped for the Wrst time using a newly developed Xuorescent
labelling strategy, referred to as A/T labelling.
The Wnal map contains 3,258 markers spanning 1,081
centiMorgans (cM) with an average distance between
two adjacent loci of 0.33 cM. This is the highest density
of markers reported for a barley genetic map to date.
The consensus map was divided into 210 BINs of about
5 cM each in which were placed 19 quantitative trait
loci (QTL) contributing to the partial resistance to barley
leaf rust (Puccinia hordei Otth) in Wve of the integrated
populations. Each parental barley combination segregated
for diVerent sets of QTLs, with only few QTLs
shared by any pair of cultivars. Defence gene homologues
(DGH) were identiWed by tBlastx homology to
known genes involved in the defence of plants against
microbial pathogens. Sixty-three DGHs were located
into the 210 BINs in order to identify candidate genes
responsible for the QTL eVects. Eight BINs were cooccupied
by a QTL and DGH(s). The positional
candidates identiWed are receptor-like kinase, WIR1
homologues and several defence response genes like
peroxidases, superoxide dismutase and thaumatin.
 
Publisher Springer Verlag
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/3001/1/A_high-density_consensus_map_of_barley_to_compare_the_distribution_of_QTLs_for_partial_resistance_to_Puccinia_hordei_and_of_defence_gene_homologues.pdf
Marcel, T C and Varshney, R K and Barbieri, M and Jafary, H and Kock, M J D de and Graner, A and Niks, R E (2007) A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues. TAG Theoretical and Applied Genetics, 114 (3). pp. 487-500. ISSN 1432-2242