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Dissection of the Barley 2L1.0 Region Carrying the ‘Laevigatum’ Quantitative Resistance Gene to Leaf Rust Using Near-Isogenic Lines (NIL) and subNIL

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Relation http://oar.icrisat.org/3006/
http://dx.doi.org/10.1094/MPMI-20-12-1604
 
Title Dissection of the Barley 2L1.0 Region Carrying the ‘Laevigatum’ Quantitative Resistance Gene to Leaf Rust Using Near-Isogenic Lines (NIL) and subNIL
 
Creator Marcel, T C
Aghnoum, R
Durand, J
Varshney, R K
Niks, R E
 
Subject Plant Pathology
Agriculture-Farming, Production, Technology, Economics
 
Description Partial resistance to leaf rust (Puccinia hordei G. H. Otth)
in barley is a quantitative resistance that is not based on
hypersensitivity. This resistance hampers haustorium formation,
resulting in a long latency period in greenhouse
tests. The three most consistent quantitative trait loci
(QTL) uncovered in the L94 × ‘Vada’ mapping population
were introgressed by marker-assisted backcrossing
into the susceptible L94 background to obtain near-isogenic
lines (NIL). We also developed the reciprocal Vada-
NIL for the susceptibility alleles of those QTL. The QTL
Rphq2 affected latency period of P. hordei more than the
QTL Rphq3 and Rphq4. The NIL confirmed the contribution
of Rphq2 to partial resistance by prolonging the latency
period by 28 h on L94-Rphq2 and shortening the
latency period by 23 h on Vada-rphq2. On the basis of
flanking restriction fragment length polymorphism-based
markers, Rphq2 appeared to be located near the telomeric
end of the long arm of chromosome 2H, in a physical
region of high recombination, making it the target QTL
for map-based cloning. Microscopic observations on the
NIL confirmed the nonhypersensitive nature of the resistance
conferred by Rphq2. A high-resolution genetic map
of the Rphq2 region was constructed using a population
of 38 subNIL with overlapping L94 introgressions in Vada
background across the region. Rphq2 mapped approximately
2 centimorgans (cM) proximal from the
MlLa locus. By bulked segregant analysis and use of
synteny with rice, we developed additional markers and
fine-mapped Rphq2 to a genetic interval of 0.11 cM that
corresponds to a stretch of sequence of, at most, 70 kb in
rice. Analysis of this rice sequence revealed predicted
genes encoding two proteins with unknown function,
retrotransposon proteins, peroxidase proteins, and a protein
similar to a mitogen-activated protein kinase kinase
kinase (MAP3K). Possible homologs of those peroxidases
and MAP3K in barley are candidates for the gene that
contributes to partial resistance to P. hordei.
 
Publisher The American Phytopathological Society
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/3006/1/Dissection_of_the_barley_2L1.0_region_carrying_the_%27Laevigatum%27_quantitative_resistance_gene_to_leaf_rust_using_near-isogenic_lines_%28NIL%29_and_subNIL.pdf
Marcel, T C and Aghnoum, R and Durand, J and Varshney, R K and Niks, R E (2007) Dissection of the Barley 2L1.0 Region Carrying the ‘Laevigatum’ Quantitative Resistance Gene to Leaf Rust Using Near-Isogenic Lines (NIL) and subNIL. Molecular Plant-Microbe Interactions, 20 (12). pp. 1604-1615. ISSN 0894-0282