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Molecular Tools for Genebank Management and Evaluation

OAR@ICRISAT

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Relation http://oar.icrisat.org/3021/
http://www.cazv.cz/static.asp?str=ProceedubgContents&ch=54&ids=2935
 
Title Molecular Tools for Genebank Management and Evaluation
 
Creator Borner, A
Roder, M S
Chebotar, S
Varshney, R K
Weinder, A
 
Subject Wheat
Agriculture-Farming, Production, Technology, Economics
 
Description Molecular markers were developed for many species and enabled us to use them for the characterisation
of genebank collections. We used the marker technology (microsatellites) for studying the genetic integrity
of the self pollinating species wheat (Triticum aestivum L.) and the open pollinating species rye (Secale cereale L.).
The study became possible, because at IPK both the ex situ collection, consisting of seeds from the most recent
regeneration and a herbarium collection is maintained. In the herbarium collection from each accession samples
of grains and complete spikes are deposited as vouchers when they are grown initially. For the wheat accessions
investigated the comparison of the DNA fingerprints showed a high degree of idendity. No contamination due
to foreign pollen or incorrect handling during the multiplication cycles was discovered. For the open pollinating
species rye, however, major changes in allele frequencies were detected. Overall, nearly 50% of the alleles discovered
in the original sample were not found in the material present in the ex situ collection now. In some cases
alleles were detected in the most recently propagated subpopulations that were not observed in the investigated
plants of the original one. In addition to the integrity studies we are in process of utilizing molecular markers
for a marker assisted screening of genebank collections. Salt tolerance of barley has been shown as a case study
in the present article.
 
Publisher Czech Academy of Agricultural Sciences
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/3021/1/Molecular_Tools_for_Genebank_Management_and_Evaluation.pdf
Borner, A and Roder, M S and Chebotar, S and Varshney, R K and Weinder, A (2005) Molecular Tools for Genebank Management and Evaluation. Czech Journal of Genetics and Plant Breeding(Special Issue), 41. pp. 122-127. ISSN 1212-1975