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Development of ESTs from chickpea roots and their use in diversity analysis of the Cicer genus

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Relation http://oar.icrisat.org/3169/
http://dx.doi.org/10.1186/1471-2229-5-16
 
Title Development of ESTs from chickpea roots and their use in diversity analysis of the Cicer genus
 
Creator Buhariwalla, H K
Jayashree, B
Eshwar, K
Crouch, J H
 
Subject Chickpea
 
Description Background: Chickpea is a major crop in many drier regions of the world where it is an important protein-rich
food and an increasingly valuable traded commodity. The wild annual Cicer species are known to possess unique
sources of resistance to pests and diseases, and tolerance to environmental stresses. However, there has been
limited utilization of these wild species by chickpea breeding programs due to interspecific crossing barriers and deleterious linkage drag. Molecular genetic diversity analysis may help predict which accessions are most likely to produce fertile progeny when crossed with chickpea cultivars. While, trait-markers may provide an effective tool for breaking linkage drag. Although SSR markers are the assay of choice for marker-assisted selection of specific traits in conventional breeding populations, they may not provide reliable estimates of interspecific diversity, and may lose selective power in backcross programs based on interspecific introgressions. Thus, we have pursued the development of gene-based markers to resolve these problems and to provide candidate gene markers for QTL mapping of important agronomic traits.
Results: An EST library was constructed after subtractive suppressive hybridization (SSH) of root tissue from
two very closely related chickpea genotypes (Cicer arietinum). A total of 106 EST-based markers were designed
from 477 sequences with functional annotations and these were tested on C. arietinum. Forty-four EST markers
were polymorphic when screened across nine Cicer species (including the cultigen). Parsimony and PCoA analysis
of the resultant EST-marker dataset indicated that most accessions cluster in accordance with the previously
defined classification of primary (C. arietinum, C. echinospermum and C. reticulatum), secondary (C. pinnatifidum, C.bijugum and C. judaicum), and tertiary (C. yamashitae, C. chrossanicum and C. cuneatum) gene-pools. A large proportion of EST alleles (45%) were only present in one or two of the accessions tested whilst the others were
represented in up to twelve of the accessions tested.
Conclusion: Gene-based markers have proven to be effective tools for diversity analysis in Cicer and EST diversity analysis may be useful in identifying promising candidates for interspecific hybridization programs. The EST markers generated in this study have detected high levels of polymorphism amongst both common and rare alleles. This suggests that they would be useful for allele-mining of germplasm collections for identification of candidate accessions in the search for new sources of resistance to pests / diseases, and tolerance to abiotic stresses
 
Publisher BioMed Central
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/3169/1/BMC%20Plant%20Biology%205_16_14p.pdf
Buhariwalla, H K and Jayashree, B and Eshwar, K and Crouch, J H (2005) Development of ESTs from chickpea roots and their use in diversity analysis of the Cicer genus. BMC Plant Biology, 5 (16). pp. 1-14. ISSN 1471-2229