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Management of late leaf spot of groundnut (Arachis hypogaea) with chlorothalonil-tolerant isolates of Pseudomonas aeruginosa

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Relation http://oar.icrisat.org/3221/
http://dx.doi.org/10.1111/j.1365-3059.2005.01160.x
 
Title Management of late leaf spot of groundnut (Arachis hypogaea) with chlorothalonil-tolerant isolates of Pseudomonas aeruginosa
 
Creator Kishore, G K
Pande, S
Podile, A R
 
Subject Groundnut
 
Description Fifteen groundnut-associated bacterial isolates that inhibited by >90% the in vitro conidial germination of Phaeoisariopsis personata, causal agent of late leaf spot disease of groundnut, were applied as a prophylactic spray (108 cfu mL-1) and tested for control of the disease in the glasshouse. Two groundnut seed-associated bacterial isolates, GSE 18 and GSE 19, identified as Pseudomonas aeruginosa, reduced the lesion frequency (LF) by up to 70%. A 90-day-old peat-based formulation of P. aeruginosa GSE 18 reduced LF measured 15 days postinoculation by up to 60%. Both P. aeruginosa GSE 18 and GSE 19 were tolerant to chlorothalonil (Kavach®) up to 2000 µg mL-1 in LB broth. In glasshouse trials, GSE 18 and GSE 19 tested in combination with reduced concentrations of chlorothalonil were highly efficient in management of the disease. The disease was completely controlled by chlorothalonil (>250 µg mL-1), and in the presence of GSE 18 or GSE 19, 100 µg mL-1 chlorothalonil was equally effective. Application of rifamycin-resistant mutants of GSE 18 or GSE 19 together with chlorothalonil significantly increased the survival of these isolates in the groundnut phylloplane. In the field, a combination of GSE 18 and 500 µg mL-1 chlorothalonil reduced disease severity comparable to 2000 µg mL-1 chlorothalonil alone. Use of chlorothalonil-tolerant pseudomonads together with a quarter concentration of the recommended field dose of chlorothalonil doubled pod yield compared with the untreated unsprayed control
 
Publisher Blackwell Publishing
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/3221/1/PlantPathology_54_3_401-408_2005.pdf
Kishore, G K and Pande, S and Podile, A R (2005) Management of late leaf spot of groundnut (Arachis hypogaea) with chlorothalonil-tolerant isolates of Pseudomonas aeruginosa. Plant Pathology, 54 (3). pp. 401-408. ISSN 0032-0862