ICAR Research Data Repository for Knowledge Management
Genetic transformation of pigeonpea with rice chitinase gene
OAR@ICRISAT
View Archive Info| Field | Value | |
| Relation |
http://oar.icrisat.org/3300/
http://dx.doi.org/10.1111/j.1439-0523.2004.01028.x |
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| Title |
Genetic transformation of pigeonpea with rice chitinase gene
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| Creator |
Kumar, S M
Kumar, B K Sharma, K K Devi, P |
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| Subject |
Pigeonpea
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| Description |
With a long-term plan to develop transgenic pigeonpea with resistance to fungal disease, the transfer of a rice chitinase gene to pigeonpea[Cajanus cajan (L.) Millsp.] is reported here. The rice chitinase gene harboured in the plasmid pCAMBIA 1302:RChit was delivered via the Agrobacterium-mediated method to the cotyledonary node explants followed by subsequent regeneration of complete plants on selection media containing hygromycin. Putative transformed pigeonpea plants were recovered with stringent selection pressure and confirmed using molecular techniques. Stable integration and expression of the chitinase gene has been confirmed in the T0 and T1 transgenics through molecular analysis. |
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| Publisher |
Blackwell Publishing
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| Date |
2004
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://oar.icrisat.org/3300/1/Genetic_transformation_of_pigeonpea_with_rice_chitinase_gene.pdf
Kumar, S M and Kumar, B K and Sharma, K K and Devi, P (2004) Genetic transformation of pigeonpea with rice chitinase gene. Plant Breeding, 123 (5). pp. 485-489. |
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