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Factors Influencing Nitrogenase Activity (Acetylene Reduction) by Root Nodules of Groundnut, Arachis hypogaea L.

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Relation http://oar.icrisat.org/3378/
http://dx.doi.org/10.3146/i0095-3679-10-1-8
 
Title Factors Influencing Nitrogenase Activity (Acetylene Reduction) by Root Nodules of Groundnut, Arachis hypogaea L.
 
Creator Nambiar, P T C
Dart, P J
 
Subject Groundnut
 
Description Acetylene reduction assay, used to measure nitrogenase activity of legume root nodules, is influenced by environmental factors, which limit its application. The effects of some of the environmental factors on acetylene reduction by groundnut root nodules are described. The activity was nonlinear during the first hour of incubation. Assay temperature above 25 C decreased the activity. Washing the nodulated roots prior to the assay also decreased the activity. The activity was influenced by light intensity, soil moisture, and moisture content in the incubation bottle. Diurnal fluctuation with one maximum and one minimum activity period during a 24 hour cycle was observed. Nitrogenase activity was higher during the postrainy season compared to that of the rainy season. A virginia cultivar Kadiri-71 had higher nitrogenase activity than a dwarf valencia cultivar, MH 2.
 
Date 1983
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/3378/1/JA_264.pdf
Nambiar, P T C and Dart, P J (1983) Factors Influencing Nitrogenase Activity (Acetylene Reduction) by Root Nodules of Groundnut, Arachis hypogaea L. Peanut Science, 10 (1). pp. 26-29.