ICAR Research Data Repository for Knowledge Management
Counting Cicer-Rhizobium using a plant infection technique
OAR@ICRISAT
View Archive Info| Field | Value | |
| Relation |
http://oar.icrisat.org/3398/
http://dx.doi.org/10.1016/0038-0717(84)90059-2 |
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| Title |
Counting Cicer-Rhizobium using a plant infection technique
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| Creator |
Toomsan, B
Rupela, O P Mittal, S Dart, P J Clark, K W |
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| Subject |
Food legumes
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| Description |
Chickpea (Cicer arietinum L.), a large seeded pulse legume, is nodulated only by a very specific group of Rhizobium strains which do not nodulate plants other than Cicer spp. If the cotyledons are removed just after germination, subsequent seedling growth is dwarfed, and plants will reliably grow and nodulate in large test tubes (25 × 200 mm) under axenic conditions with either sand or sand + venniculite as the root medium. The dwarfed seedlings of some selected chickpea lines can be used as a “trap host” for counting Cicer-Rhizobium in pure cultures and contaminated materials by a most probable number, serial dilution-plant infection technique. The value of such a plantlet as a “trap host” for studying Cicer-Rhizobium ecology, strain authentication and inoculum quality control is demonstrated.
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| Publisher |
Pergamon
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| Date |
1984
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://oar.icrisat.org/3398/1/JA_304.pdf
Toomsan, B and Rupela, O P and Mittal, S and Dart, P J and Clark, K W (1984) Counting Cicer-Rhizobium using a plant infection technique. Soil Biology and Biochemistry, 16 (5). pp. 503-507. ISSN 0038-0717 |
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