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Effect of Various Stains on The Stability And Particle Length Distribution of Three Poty Viruses

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Title Effect of Various Stains on The Stability And Particle Length Distribution of Three Poty Viruses
 
Creator Manohar, S K
Rajeshwari, R
 
Subject Plant Virology
Fertilizer Appications
 
Description Four negative stains were tested on Peanut mottle virus (PMV), two irolater of Peanut
green mosaic virus-systemic necrosis (PGMV-SN) ar~dsy etrmic mosaic (PGM V-SM), to datrrmlne
their effects on the stability and particle length distribution.
Whan phosphotunngstate(PleTA ) at pH 7.0 was used, particle lengh and width of PMV
and PGMV-SM werr conside~ably affecrnd rasulting in variation, whereas PGMV-SN war la18
affected. When uranyl acotete (UA) and urrnyl formate (UF) ware ured, tho prrlicler of
PGMV-SM and PMV were better preserved, in contrast to PGMV-SN which was unstable in
both the uranyl stains. PGMV.SN was well presarved in ammonium molybdrte (AM) o f pHs
3.5 and 6.5 but was d;sruoted in OH 8.0. Although PMV could be stairled with AM of pH
3.6, particles ware poorly stained In wM ot pHs 6.6 and 8.0. Pertlcles ol PGMV-SM were
poorly strined in AM of all the three pH valuer.
 
Publisher Indian Virological Society
 
Date 1985
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/3510/1/JA_531.pdf
Manohar, S K and Rajeshwari, R (1985) Effect of Various Stains on The Stability And Particle Length Distribution of Three Poty Viruses. Indian Journal of Virology, 1 (2). pp. 157-162. ISSN 0974-0120