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Detection of a mycoplasma-like organism in peanut plants with witches' broom using indirect enzyme-linked immunosorbent assay (ELISA)

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Relation http://oar.icrisat.org/3595/
http://dx.doi.org/10.1111/j.1365-3059.1987.tb02216.x
 
Title Detection of a mycoplasma-like organism in peanut plants with witches' broom using indirect enzyme-linked immunosorbent assay (ELISA)
 
Creator Hobbs, H A
Reddy, D V R
Reddy, A S
 
Subject Groundnut
 
Description The mycoplasma-like organism (MLO) associated with peanut (groundnut) witches’ broom (PWB) from India was partially purified and an antiserum produced against it. Using a protein A indirect enzyme-linked immunosorbent assay (ELISA) procedure, PWB MLO was detected in crude extracts of leaves. stems and pegs of infected peanut plants, although stems were a better source than leaves and pegs. Extracts of infected tissues of three diseases of assumed MLO etiology in India, little leaf of brinjal (eggplant). I med rosed witches “broom, and Daturd sp. witches’ broom, failed to react with the PWB MLO antiserum.
 
Publisher Blackwell Publishing
 
Date 1987
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/3595/1/JA_613.pdf
Hobbs, H A and Reddy, D V R and Reddy, A S (1987) Detection of a mycoplasma-like organism in peanut plants with witches' broom using indirect enzyme-linked immunosorbent assay (ELISA). Plant Pathology, 36 (2). pp. 164-167. ISSN 00320862