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In vitro Culture of Ovules and Embryos from some Incompatible Interspecific Crosses in the Genus Arachis L.

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Title In vitro Culture of Ovules and Embryos from some Incompatible Interspecific Crosses in the Genus Arachis L.
 
Creator Mallikarjuna, N
Sastri, D C
 
Subject Groundnut
 
Description In vrtro culture of ovules and embyros IS commonly used to produce hybrrds from rncompalrble
crosses. In several mlerspecrlrc crossar in Arachlr, ovule and or embryo cullure rs necessary
because the ovules do nor develop lully in vrvo
Ovule srzes from drfferml crosses and dliierenr hormone lrealmenrs range lrom less then 0 3 mm
lo 5 0 mm, rarely 7 0 mm Ovules smaller than 3 0 mm had to be cultured mlrre as the proembryos
rhey conlamed were roo small lor drsseclron and cullure Ovules larger rhan 3 Omm were drssecred
and lherr embryos cullured
All the ovules were culturedon MS medra with or wrlhoul agar and with diilerenl concenlrrtrons
of various hormones
Dependrng on the hormones used and therr concenlratrons, drilerenr responses were observed
Surface callus formatron, greenmg, swellrng. and browntng were common rn cullured ovules
Embryos emerged from some of the ovules end planllers have been oblarned Cultured embryos
showed srmrler responses, and some cultured embryos germinated, and developed rnlo planllsls
 
Date 1985
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/4159/1/CP_206.pdf
Mallikarjuna, N and Sastri, D C (1985) In vitro Culture of Ovules and Embryos from some Incompatible Interspecific Crosses in the Genus Arachis L. In: Proceedings of an International Workshop on Cytogenetics of Arachis, 31 Oct - 2 Nov 1983, Patancheru, Andhra Pradesh, India.