Record Details

Potential of Enzyme-linked Immunosorbent Assay for Detecting Viruses, Fungi, Bacteria, Mycoplasma-like Organisms, Mycotoxins, and Hormones

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Relation http://oar.icrisat.org/4447/
 
Title Potential of Enzyme-linked Immunosorbent Assay for Detecting Viruses, Fungi, Bacteria, Mycoplasma-like Organisms, Mycotoxins, and Hormones
 
Creator Reddy, D V R
Nambiar, P T C
Rajeswari, R
Mehan, V K
Anjaiah, V
McDonald, D
 
Subject Genetics and Genomics
Plant Pathology
 
Description Enz-vme-linked immunosorbent assay (ELlSA) is one of the most widely employed serological
tests. For example, the double antibody sandwich (DAS-ELlSA) technique has been successfully
employed for the detection of various rhizobia, to assess the quality of inoculum, and to assess the
establishment of the inoculated rhizobial strains in soil. This technique can also distinguish
bet ween different strains o fR hi~obiumE. LISA has successfully been adapted to direct antigen
coating (DAC-ELISA) and protein A coating (PAC) procedures for the detection and assay of
fveralpeanut viruses. DAC-ELlSA is a simple and useful tool for virus detection in field surveys
hd in seed samples. Both DA C- and PA C- ELISA are more convenient to use lbr the detection of
large numbers of antigens than DAS-EL ISA. More recently, a competitive ELISA procedure has
been developed to detect up to 2 ng aflatoxin B, in goundnut seed samples.
 
Date 1988
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/4447/1/CP_391.pdf
Reddy, D V R and Nambiar, P T C and Rajeswari, R and Mehan, V K and Anjaiah, V and McDonald, D (1988) Potential of Enzyme-linked Immunosorbent Assay for Detecting Viruses, Fungi, Bacteria, Mycoplasma-like Organisms, Mycotoxins, and Hormones. In: Biotechnology in tropical crop improvement: proceedings of the International Biotechnology Workshop, 12-15 Jan 1987, ICRISAT Patacheru, India.