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Embryo Rescue in Wide Crosses in Arachis. 1. Culture of Ovules in Peg Tips of Arachis hypogaea

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Relation http://oar.icrisat.org/4466/
http://aob.oxfordjournals.org/content/61/1/1.abstract
 
Title Embryo Rescue in Wide Crosses in Arachis. 1. Culture of Ovules in Peg Tips of Arachis hypogaea
 
Creator Moss, J P
Stalker, H T
Pattee, H E
 
Subject Groundnut
 
Description Interspecific hybridization in Arachis is restricted by early embryo abortion for many cross-combinations. Rescue of young embryos in vitro within a week after fertilization is necessary to recover these embryos before they abort. Peg tips, with the ovule and embryo tissues, of A. hypogaea L. cv. ‘NC 6’, were cultured to compare ovule growth, callus production and peg elongation. Tissues were collected 1, 2, 3 and 4 d after self-pollination, after which peg meristems were removed from half the pegs and cultured on five media combinations. One-day-old pegs had significantly (P = 0.01) more ovule growth than older tissues. Presence of the meristem had a greater inhibition to ovule growth for 2- to 4-d pegs than for 1-d-old pegs. Significantly more callus was produced on 4-d pegs than younger tissues, and kinetin had the greatest stimulatory effect on callus. Elongation of pegs with the meristem attached was observed most often in media with high sucrose levels. The observations indicate that very young ovules can be grown in vitro, and techniques may be applicable to rescue of young embryonic tissues of Arachis
 
Publisher Oxford University Press
 
Date 1988
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/4466/1/AnnalsofBotany_61_1_1-7_1988.pdf
Moss, J P and Stalker, H T and Pattee, H E (1988) Embryo Rescue in Wide Crosses in Arachis. 1. Culture of Ovules in Peg Tips of Arachis hypogaea. Annals of Botany, 61 (1). pp. 1-7. ISSN 0305-7364