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Improved Screening Techniques for In-vitro Seed Colonization and Pre-harvest Seed Infection by Aspergillus flavus In Groundnut

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Relation http://oar.icrisat.org/4915/
 
Title Improved Screening Techniques for In-vitro Seed Colonization and Pre-harvest Seed Infection by Aspergillus flavus In Groundnut
 
Creator Thakur, R P
Rao, V P
Upadhyaya, H D
Nigam, S N
Talwar, H S
 
Subject Groundnut
 
Description Preharvest infection of groundnut seeds by toxigenic strains of Aspergillus flavus and A. parasiticus is a major cause for aflatoxin contamination in the crop. The objective of this study was to refine screening techniques for in-vitro seed colonization and preharvest seed infection that could be effectively used in resistance breeding programmes. A highly aggressive and toxigenic strain of A. flavus, Af 11-4, was used to improve the screening techniques for in-vitro seed colonization and preharvest seed infection. The in-vitro seed colonization technique involved the use of sodium hypochlorite (NaOCl-clorax 20%) instead of mercuric chloride (HgCl2 0.1%) or ethanol (ethyl alcohol 70%) as seed surface disinfectant, and multiwell dishes to incubate single inoculated seeds that will facilitate selection of seeds resistant to A. flavus colonization. The preharvest seed infection screening technique involved the development of A. flavus infested beds and imposing soil moisture stress (54-63% water deficit) during the last 48 days of the crop growth. Both in-vitro seed colonization and field screening methods were successfully used to screen a number of groundnut genotypes with consistent results.
 
Publisher Plant Protection Association of India
 
Date 2003
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/4915/1/GNAF%20IJPP%202003%2031%2054-60.pdf
Thakur, R P and Rao, V P and Upadhyaya, H D and Nigam, S N and Talwar, H S (2003) Improved Screening Techniques for In-vitro Seed Colonization and Pre-harvest Seed Infection by Aspergillus flavus In Groundnut. Indian Journal of Plant Protection, 31 (2). pp. 54-60. ISSN 0253-4355