Serodiagnosis of animal trypanosomosis using a recombinant invariant surface glycoprotein of Trypanosoma evansi

Abstract

Trypanosomosis is endemic in many parts of the world in animals and humans. The diagnosis of asymptomatic carrier animals and a followup treatment is essential for control of the disease. In the present study, the extracellular domain (ED) region of invariant surface glycoprotein (ISG-75) gene of Trypanosoma evansi consisting of 1320 nucleotides (nt), encoding a polypeptide of 440 amino acids (aa) has been cloned and expressed in eukaryotic system Pichia pastoris (X-33). Immunoreactivity of the expressed protein (~70 kDa) was checked using a panel of standard serum samples. Furthermore, the diagnostic potential of the protein was evaluated using bovine, camel and horse serum samples from the field. Statistical analysis of the bovine data showed optimum combination of sensitivity and specificity at 98.6 and 99.7, respectively (>0.547 cut off OD value). The present finding revealed that the recombinant protein can be exploited as a potential diagnostic antigen in the serodiagnosis of trypanosomosis.