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http://krishi.icar.gov.in/jspui/handle/123456789/12251| Title: | Association of Tomato Leaf Curl New Delhi Virus, A Bipartite Begomovirus with Mosaic Disease of Snake Gourd in India |
| Other Titles: | Not Available |
| Authors: | V. Venkataravanappa, C. N. Lakshminarayana Reddy, Neha Chauhan, D. R. Bhardwaj and M. Krishna Reddy |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | CHES, Chettalli, Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bangalore (Karnataka) Indian Institute of Vegetable Research, Varanasi-221305, (Uttar Pradesh) Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bangalore-560089, Karnataka (Karnataka) Department of Plant Pathology, College of Sericulture, University of Agricultural Sciences (B), Chintamani (Karnataka) |
| Published/ Complete Date: | 2017-05-01 |
| Project Code: | Not Available |
| Keywords: | Snake gourd, Begomovirus, PCR, Recombination, Phylogentic analysis, Tomato leaf curl New Delhi virus (ToLCNDV) |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Total twenty five snake gourd leaf samples showing severe mosaic symptoms were collected from different farmer’s field in Varanasi, Uttar Pradesh State of India. The partial amplified PCR products (1.2kb fragment) were cloned and sequence characterized. On the basis of the determined sequences and sequence analysis, begomovirus associated with symptoms in majority of samples (20) was found to be a member of a bipartite begomovirus species which is closely related to Tomato leaf curl New Delhi virus (ToLCNDV). Therefore one sample was selected for full-length amplification using RCA method. SDT analysis of complete genome of the begomovirus (SnG-1) showed highest nucleotide (nt) identity of 88.5-96.3% (DNA-A) and 82.7-93.3% (DNA-B) with Tomato leaf curl New Delhi virus (ToLCNDV) infecting different cucurbits in Indian subcontinent. An analysis for recombinant origin of genomes (DNA-A and DNA-B-like sequence) showed major part of their genome was likely, originated by recombination of previously reported begomoviruses (ToLCNDV, ToLCPaV and SLCCNV) infecting different cucurbits species resulting in evolution of new recombinant virus. This is the first report of ToLCNDV associated Snake gourd India and significance of these findings is discussed. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Pure & Applied Bioscience |
| Volume No.: | 5 (5) |
| Page Number: | 558-570 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/12251 |
| Appears in Collections: | HS-IIVR-Publication |
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