Computer-Assisted Sperm Analysis of Freezable and Nonfreezable Mithun (Bos frontalis) Semen

Abstract

The present study was undertaken to assess the motility and velocity parameters of sperm of freezable and nonfreezable ejaculates by computer-assisted spermanalyser (CASA) such as Hamilton-Thorne Semen Analyser IVOS 11 inmithun semen. Fifty ejaculates (twenty-five ejaculates each for freezable and nonfreezable semen ejaculates) were collected fromtenmaturedmithun bulls. CASA parameters,motility parameters such as forward progressivemotility (FPM) (%), nonprogressive motility (NPM) (%), total motility (TM) (%), and static sperms (SM) (%); velocity parameters such as curvilinear velocity (VCL) (𝜇m/sec), straight line velocity (VSL) (𝜇m/sec), average path velocity (VAP) (𝜇m/sec), linearity (LIN) (%), straightness (STR) (%), wobble (WOB) (%), amplitude of lateral head displacement (ALH) (𝜇m), and beat/cross-frequency (BCF) (Hz) weremeasured by CASA analyser.The result revealed that these parameters varied significantly (𝑃 < 0.05) between the freezable and nonfreezable ejaculates and freezable ejaculates have significantly (𝑃 < 0.05) higher value than nonfreezable ejaculates. It was concluded that most of the CASA parameters were significantly lower in nonfreezable ejaculates than in freezable ejaculates in mithun and confirmed that the CASA was effective for a quick and objective analysis of motility and velocity parameters in mithun semen.