KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/17518| Title: | Artificial microRNA-derived resistance to Cassava brown streak disease |
| Other Titles: | Not Available |
| Authors: | Henry Wagaba Basavaprabhu L. Patil Settumba Mukasa Titus Alicai Claude M. Fauquet Nigel J. Taylor |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | National Crops Resources Research Institute, Namulonge, P.O. Box 7084, Kampala, Uganda Makerere University, P.O. Box 7062, University Rd, Kampala, Uganda ICAR::National Research Centre on Plant Biotechnology, India Centro Internacional de Agricultura Tropical, Cali, Apartado Aéreo 6713, Cali, Colombia Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA |
| Published/ Complete Date: | 2016-06-01 |
| Project Code: | Not Available |
| Keywords: | Artificial microRNA Cassava brown streak disease |
| Publisher: | Elsevier |
| Citation: | H. Wagaba, B. L. Patil, S. Mukasa, T. Alicai, C. M. Fauquet and N. J. Taylor. (2016) Artificial microRNA-derived resistance to Cassava brown streak disease. Journal of Virological Methods. 231, 38-43. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Artificial miRNAs (amiRNA) were generated targeting conserved sequences within the genomes of the two causal agents of Cassava brown streak disease (CBSD): Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Transient expression studies on ten amiRNAs targeting 21nt conserved sequences of P1(CBSV and UCBSV), P3(CBSV and UCBSV), CI(UCBSV), NIb(CBSV and UCBSV), CP(UCBSV) and the un-translated region (3'-UTR) were tested in Nicotiana benthamiana. Four out of the ten amiRNAs expressed the corresponding amiRNA at high levels. Transgenic N. benthamiana plants were developed for the four amiRNAs targeting the P1 and NIb genes of CBSV and the P1 and CP genes of UCBSV and shown to accumulate miRNA products. Transgenic plants challenged with CBSV and UCBSV isolates showed resistance levels that ranged between ∼20-60% against CBSV and UCBSV and correlated with expression levels of the transgenically derived miRNAs. MicroRNAs targeting P1 and NIb of CBSV showed protection against CBSV and UCBSV, while amiRNAs targeting the P1 and CP of UCBSV showed protection against UCBSV but were less efficient against CBSV. These results indicate a potential application of amiRNAs for engineering resistance to CBSD-causing viruses in cassava. |
| Description: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Virological Methods |
| NAAS Rating: | 7.79 |
| Volume No.: | 231 |
| Page Number: | 38-43 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | http://dx.doi.org/10.1016/j.jviromet.2016.02.004 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/17518 |
| Appears in Collections: | HS-IIHR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Wagaba et al-Artificial microRNA-derived resistance to CBSD-2016.pdf | 1.34 MB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.