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http://krishi.icar.gov.in/jspui/handle/123456789/19811| Title: | One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle |
| Other Titles: | Not Available |
| Authors: | Wiebke Garrels1,2,*, Thirumala R. Talluri1,*,†, Ronja Apfelbaum1, Yanet P. Carratalá1,3, Pablo Bosch4, Kerstin Pötzsch5, Esther Grueso5, Zoltán Ivics5 & Wilfried A. Kues1 |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR-NRCE 1Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Neustadt, Germany 2Institute for Laboratory Animal Sciences, Medical School Hannover (MHH), Germany. Biotechnology Department, Center for Genetic Engineering and Biotechnology, Havana, Cuba. 4Departamento de Biología Molecular, FCEFQyN, Universidad Nacional de Río Cuarto, Córdoba, Argentina. 5Paul-Ehrlich-Institute, Langen, Germany |
| Published/ Complete Date: | 2016-02-24 |
| Project Code: | Not Available |
| Keywords: | transgenesis, cattle, sleeping beauty, piggybac |
| Publisher: | Nature.com |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we established a highly efficient one-step multiplex gene transfer system into the bovine genome. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Language: | English |
| Name of Journal: | Scientific Reports |
| NAAS Rating: | 10 |
| Volume No.: | Scientific Reports | 6:21953 | DOI: 10.1038/srep21953 |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Dept Biotechnology |
| Source, DOI or any other URL: | doi: 10.1038/srep21953 (2016). |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/19811 |
| Appears in Collections: | AS-NRCE-Publication |
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