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Title: | Isolation and Characterization of a Non-Saccharomyces Yeast with Improved Functional Characteristics for Ethanol Production |
Other Titles: | Not Available |
Authors: | Kaur S, Oberoi HS, Phutela R |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Institute of Horticultural Research Punjab Agricultural University Ludhiana ICAR-Central Institute of Post Harvest Engineering and Technology |
Published/ Complete Date: | 2018-02-18 |
Project Code: | Not Available |
Keywords: | Ethanol productivity; glucose concentration; non-Saccharomyces yeasts; Pichia kudriavzevii; thermotolerant yeasts |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Aim: To isolate a thermotolerant yeast strain with fermentation potential at elevated temperatures. Study Design: Lab experimental design was used in the study.Methodology: Yeasts were isolated from over-ripened fruits and naturally fermenting sugarcane juice. Four isolates showing relatively higher fermentation ability were screened for their fermentation potential. The selected strain was tested for its thermotolerance, osmotolence as well as to work at varied pH. Results: Isolate Y-4 produced relatively higher ethanol than the other isolates from 15 gl-1 glucose. Scanning electron micrographs (SEM) of isolate Y-4 showed oval to spherical cells with diameter ranging from 4.5 to 6.2 μm. On the basis of the SEM images and 28s rRNA gene sequencing isolate Y-4 was identified as Pichia kudriavzevii and designated as P. kudriavzevii SK1. P.kudriavzevii SK1 metabolized glucose, galactose, mannose, maltose and fructose. It showed the potential to grow at a glucose concentration of 300 gl-1 and ferment at 45°C, though the best results were obtained from 15-20 gl-1 glucose at 35°C. Reference strain Saccharomyces cerevisiae MTCC 11815 produced low concentrations of ethanol under similar conditions. With 200 gl-1 initial glucose concentration 86.1 and 87.9 gl-1 ethanol was obtained in shake flasks and laboratory batch fermenter experiments, respectively at pH 5 at 35°C . This study revealed that P. kudriavzevii SK1 could be utilized for pilot scale fermentation for high gravity fermentations. |
Description: | Not Available |
ISSN: | 2231-0886 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Microbiology Research Journal International |
Volume No.: | 26 |
Page Number: | 1-9 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://dx.doi.org/10.9734/MRJI/2018/v26i430073 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/23503 |
Appears in Collections: | HS-IIHR-Publication |
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