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http://krishi.icar.gov.in/jspui/handle/123456789/24634| Title: | Cloning and expression of Toxocara canis arginine kinase recombinant protein for serodetection of toxocarosis in dogs |
| Other Titles: | Not Available |
| Authors: | Varsha Bagde, Siju Susan Jacob, S. Samanta, Shivani Sahu, N.R., Sudhakar, J.K. Chamuah, Harikesh Singh Yadav, Rajat Garg and O.K. Raina* |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Research Centre on Mithun |
| Published/ Complete Date: | 2013-01-01 |
| Project Code: | Not Available |
| Keywords: | Toxocara canis, Dog, Arginine kinase, Recombinant antigen, ELISA, Serodiagnosis. |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Toxocarosis is a widespread zoonosis caused by the ascarid Toxocara canis. Serodiagnosis of toxocarosis is carried out using Toxocara excretory-secretory and recombinant antigens. In the present study T. canis arginine kinase gene was amplified by polymerase chain reaction, cloned in a prokaryotic expression vector and expressed in Escherichia coli. The recombinant protein was used in IgG-ELISA for detection of T. canis infection in the adult dogs. Further evaluation of this recombinant antigen in the diagnosis of T. canis infection in dogs is under way. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Not Available |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/24634 |
| Appears in Collections: | AS-NRCM-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| varsha paper-Jayanta.pdf | 333.65 kB | Adobe PDF | View/Open |
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