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http://krishi.icar.gov.in/jspui/handle/123456789/26058| Title: | Detection of viruses infecting black pepper by SYBR Green-based real-time PCR assay |
| Other Titles: | Not Available |
| Authors: | Bhat, A.I. and Siljo, A. 2014. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Spices Research |
| Published/ Complete Date: | 2014 |
| Project Code: | BT/PB/03/01/2007 |
| Keywords: | Cucumber mosaic virus Piper yellow mottle virus Real-time PCR Real-time RT-PCR Detection Sensitivity |
| Publisher: | Italian Phytopathological Society |
| Citation: | Bhat, A.I. and Siljo, A. 2014. Detection of viruses infecting black pepper by SYBR Green-based real-time PCR assay. Journal of Plant Pathology, 96: 105-109. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Virus infections are a major constraint to cultivation of black pepper (Piper nigrum L.), a perennial vegetatively propagated spice crop. Real-time PCR and real-time RTPCR were developed to detect Piper yellow mottle virus (PYMoV) and Cucumber mosaic virus (CMV), respectively, in black pepper plants. These detection assays were up to 1000 times more sensitive than the conventional PCR/RTPCR assays. Field samples from different pepper-growing regions in India were used to validate the two detection assays, which identified more infected plants than conventional PCR. Our results indicate that real-time PCR should be the method of choice for screening mother plants for PYMoV and CMV. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Plant Pathology |
| NAAS Rating: | 7.15 |
| Volume No.: | 96 (1) |
| Page Number: | 105-109 |
| Name of the Division/Regional Station: | Crop Protection |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/26058 |
| Appears in Collections: | HS-IISR-Publication |
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