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http://krishi.icar.gov.in/jspui/handle/123456789/31819| Title: | Development of naive phage display VHH libraries from Indian camel |
| Other Titles: | Not Available |
| Authors: | Lipsa Dash Saravanan Subramaniam S.A. Khulape B.R. Prusty Kamal Pargai Shirish Dadarao Narnaware Niteen V Patil B. Pattnaik |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Research Centre on Camel |
| Published/ Complete Date: | 2016-01-01 |
| Project Code: | Not Available |
| Keywords: | Nano body phagemid Redundancy Single pot library |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The present study describes the construction of naïve camelid VHH single pot library from lymphocytes of non-immunized Camelus dromedarius. RNA extracted from 6 healthy female camels was pooled together and used for construction of library in phagemid vector. The VHH inserts were found in 50-70% of the phagemid vector (pMECS vector) cloned in Escherichia coli and the size of the libraries was estimated to be around 10⁵-10⁷cfu. Sequence analysis of VHHs revealed variations in the length of complementary determining regions and amino acid usage resulting in a variety of different paratopes. Further expression of VHH rescued from the library was optimized in prokaryotic system using pET32 expression system. Functional antibodies generated from this naïve library will be explored for affinity against various antigens in further studies. © 2016, Indian Council of Agricultural Research. All rights reserved. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Animal Sciences |
| NAAS Rating: | 6.28 |
| Volume No.: | 86 |
| Page Number: | 857-860 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/31819 |
| Appears in Collections: | AS-NRCC-Publication |
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