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Title: | Simultaneous direct analysis of aflatoxins and ochratoxin A in cereals and their processed products by ultra-high performance liquid chromatography with fluorescence detection |
Other Titles: | Not Available |
Authors: | Dhanshetty M, Banerjee Kaushik |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Research Centre for Grapes |
Published/ Complete Date: | 2019-11-01 |
Project Code: | Not Available |
Keywords: | Aflatoxins Ochratoxin A Cereals Processed products Ultra-High Performance Liquid Chromatography with Fluorescence Detection |
Publisher: | Oxford University Press |
Citation: | Dhanshetty M, Banerjee Kaushik (2019). Simultaneous direct analysis of aflatoxins and ochratoxin A in cereals and their processed products by ultra-high performance liquid chromatography with fluorescence detection. Journal of AOAC International 102 (6): 1666-1672. DOI: https://doi.org/10.1093/jaoac/102.6.1666 |
Series/Report no.: | Not Available; |
Abstract/Description: | Background: Mycotoxins such as aflatoxins (AFs) and ochratoxin A (OTA) can pose severe health hazards because of their toxicity. Given a wide range of food matrices susceptible to fungal infections and possible cooccurrence of mycotoxins at different concentrations, validated multimycotoxin and multimatrix methods are strongly warranted. Objective: The aim of this research was to develop a simple and fast ultra-high performance LC (UHPLC) fluorescence detection (FLD)–based method to simultaneously determine AFs (B1, G1, B2, and G2) and OTA and, furthermore, to carry out single-laboratory validation in a range of cereals and processed product matrices. Methods: The sample preparation involved homogenization and extraction with methanol–water (80 + 20). For cleanup, an aliquot (3 mL) was diluted with phosphate-buffered saline, loaded on an immunoaffinity column (AFLAOCHRA PREP®), and eluted with methanol (1 mL). The cleaned extract was diluted with 0.2% acetic acid (at a 1:1 ratio) before injection into an ultra-high performance liquid chromatograph. To perform simultaneous analysis of AFs and OTA, the FLD program was developed by switching the excitation wavelength in a single chromatographic run. Results: The method provided LOQs of 0.25 and 1 ng/g for AFs and OTA, respectively, without involving any derivatization. In rice, the recoveries of AFs ranged from 84 to 106%, whereas OTA had a recovery above 72%, with the repeatability relative SDs <12% for both analytes. The method was successfully applied to a range of naturally contaminated market samples. Conclusions: The method is suitable for regulatory testing because of its significant time and cost effectiveness and sensitivity in compliance with the regulatory maximum levels. |
Description: | Not Available |
Type(s) of content: | Journal |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of AOAC International |
Volume No.: | 102 |
Page Number: | 1666–1672 |
Name of the Division/Regional Station: | National Reference Laboratory |
Source, DOI or any other URL: | https://doi.org/10.1093/jaoac/102.6.1666 |
URI: | https://doi.org/10.1093/jaoac/102.6.1666 http://krishi.icar.gov.in/jspui/handle/123456789/38647 |
Appears in Collections: | HS-NRCG-Publication |
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