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http://krishi.icar.gov.in/jspui/handle/123456789/41132| Title: | De Novo Assembly of Bitter Gourd Transcriptomes: Gene Expression and Sequence Variations in Gynoecious and Monoecious Lines |
| Other Titles: | Not Available De Novo Transcriptomes Assembly of Bitter Gourd |
| Authors: | Not AvailaAnjali Shukla, V. K. Singh, D. R. Bharadwaj, Rajesh Kumar, Ashutosh Rai, A. K. Rai, Raja Mugasimangalam, Sriram Parameswaran, Major Singh, P. S. Naikble |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Vegetable Research, Centre for Bioinformatics, School of Biotechnology, Banaras Hindu University, Centre of Advanced Studies, Department of Botany, Banaras Hindu University, Genotypic Technology (P) Ltd., Bangalore |
| Published/ Complete Date: | 2015-06-05 |
| Project Code: | Not Available |
| Keywords: | Bitter Gourd, Transcriptome, Gynoecious and Monoecious |
| Publisher: | PLOS ONE |
| Citation: | Shukla Anjali, Singh VK, Bharadwaj DR, Kumar Rajesh, Rai Ashutosh, Rai AK, Mugasimangalam Raja, Parameswaran Sriram, Singh Major, Naik PS (2015). De novo assembly of bitter gourd transcriptomes: gene expression and sequence variations in gynoecious and monoecious lines. PLoS ONE 10(6): e0128331. doi:10.1371/journal.pone.0128331. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Bitter gourd (Momordica charantia L.) is a nutritious vegetable crop of Asian origin, used as a medicinal herb in Indian and Chinese traditional medicine. Molecular breeding in bitter gourd is in its infancy, due to limited molecular resources, particularly on functional markers for traits such as gynoecy. We performed de novo transcriptome sequencing of bitter gourd using Illumina next-generation sequencer, from root, flower buds, stem and leaf samples of gynoecious line (Gy323) and a monoecious line (DRAR1). A total of 65,540 transcripts for Gy323 and 61,490 for DRAR1 were obtained. Comparisons revealed SNP and SSR variations between these lines and, identification of gene classes. Based on available transcripts we identified 80 WRKY transcription factors, several reported in responses to biotic and abiotic stresses; 56 ARF genes which play a pivotal role in auxin-regulated gene expression and development. The data presented will be useful in both functions studies and breeding programs in bitter gourd. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | ICAR |
| Language: | English |
| Name of Journal: | PLOS One |
| NAAS Rating: | 8.74 |
| Volume No.: | 10 (6) |
| Page Number: | 1-19 |
| Name of the Division/Regional Station: | Division of Crop Improvement |
| Source, DOI or any other URL: | doi:10.1371/ journal.pone.0128331 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/41132 |
| Appears in Collections: | HS-IIVR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| De novo assembly of bitter gourd transcriptomes gene expression and sequence variations in gynoecious and monoecious lines.pdf | 2.82 MB | Adobe PDF | View/Open |
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