KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/4497| Title: | Application of a loop-mediated isothermal amplification assay for rapid detection of cow components adulterated in buffalo milk/meat |
| Other Titles: | Not Available |
| Authors: | Not Available Deb R, Sengar G S, Singh U, Kumar S, Raja T V, Alyethodi RR, Alex R, Das AK, Prakash B |
| Published/ Complete Date: | 2016-10-19 |
| Project Code: | Not Available |
| Keywords: | LAMP assay Cow components Adulteration Buffalo milk/meat samples |
| Publisher: | Springer |
| Citation: | Not Available |
| Abstract/Description: | Loop-mediated isothermal amplification (LAMP) is a diagnostic method for amplification of DNA with rapid and minimal equipment requirement. In the present study, we applied the LAMP assay for rapid detection of cow components adulteration in buffalo milk/ meat samples. The test can be completed within around 1 h 40 min starting from DNA extraction and can be performed in water bath without requirement of thermocycler. The cow DNA in buffalo samples were identified in the developed LAMP assay by either visualizing with SYBR Green I/HNB dyes or observing the typical ladder pattern on gel electrophoresis. The test can detect up to 5 % level of cow milk/meat mixed in buffalo counterparts. Due to the simplicity and specificity, the developed LAMP test can be easily adapted in any laboratory for rapid detection of cow species identification in livestock by products. |
| Description: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | AICRP on Cattle,ICAR |
| Language: | English |
| Name of Journal: | Molecular Biotechnology |
| NAAS Rating: | 8.02 |
| Volume No.: | 58 |
| Page Number: | 850-860 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | DOI 10.1007/s12033-016-9984-4 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/4497 |
| Appears in Collections: | AS-CIRC-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.