KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/46910| Title: | Mesenchymal stem cell‐conditioned media: A novel alternative of stem cell therapy for quality wound healing |
| Other Titles: | Not Available |
| Authors: | Anand joseph Indu Baiju Irfan A. Bhat Sriti Pandey Mukesh Bharti Megha Verma Anuj Pratap Singh Matin M. Ansari Vikash Chandra Gutulla Saikumar Amarpal Gutulla Taru Sharma |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Research Centre on Camel ICAR‐Indian Veterinary Research Institute, Izatnagar |
| Published/ Complete Date: | 2020-01-21 |
| Project Code: | Not Available |
| Keywords: | conditioned media growth factors stem cells wound healing |
| Publisher: | wiley |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Mesenchymal stem cells‐conditioned media (MSCs‐CM) contains several growth factors and cytokines, thus may be used as a better alternative to stem cell therapy, which needs to be elucidated. The present study was conducted to evaluate the therapeutic potential of caprine, canine, and guinea pig bone marrow‐derived MSCs‐CM in excision wound healing in a guinea pig model. MSCs were obtained from bone marrow, expanded ex vivo and characterized as per ISCT criteria. CM was collected assayed by western blot to ascertain the presence of important secretory biomolecules. Quantitative estimation by enzyme‐linked immunosorbent assay was done for a vascular epidermal growth factor (VEGF) and interleukin‐6 (IL‐6) in caprine MSCs‐CM and optimum time for collection of CM was decided as 72 hr. CM from all the species was lyophilized by freeze‐drying method. Full‐thickness (2 × 2 cm2) excision skin wounds were created in guinea pigs (six animals in each group) and respective lyophilized CM mixed with laminin gel was applied topically at weekly interval. On Day 28, histopathological examinations of healed skin were done by hemotoxylin and eosin staining. MSCs were found to secrete important growth factors and cytokines (i.e., VEGF, transforming growth factor‐β1, fibroblast growth factor‐2, insulin‐like growth factor‐1, stem cell factor, and IL‐6) as demonstrated by immunohistochemistry and western blot assay. It was found that allogenic and xenogenic application of CM significantly improved quality wound healing with minimal scar formation. Thus, MSCs‐CM can be used allogenically as well as xenogenically for quality wound healing. |
| Description: | Not Available |
| ISSN: | 0021-9541 |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Cellular Physiology |
| NAAS Rating: | 11.55 |
| Volume No.: | 235(7-8) |
| Page Number: | 5555-5569 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | doi.org/10.1002/jcp.29486 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/46910 |
| Appears in Collections: | AS-NRCC-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.