KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/49408| Title: | Development and application of a novel triplex-PCR assay for rapid detection of Methicillin resistant Staphylococcus aureus (MRSA) from pigs |
| Other Titles: | Not Available |
| Authors: | Rajkhowa, S., Pegu, S. R., P. S., Girish and R. Agrawal |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Research Centre on Meat |
| Published/ Complete Date: | 2020-10-01 |
| Project Code: | Not Available |
| Keywords: | triplex-PCR assay, rapid detection, methicillin-resistant, Staphylococcus aureus, pigs |
| Publisher: | Letters in Applied Microbiology |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A triplex-PCR assay was developed and evaluated for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) recovered from various biological samples of pig. Three sets of primers were designed to target mecA, 16S rRNA and nuc genes of MRSA. The specific amplification generated three bands on agarose gel, with sizes 280 bp for mecA, 654 bp for 16S rRNA and 481 bp for nuc, respectively. A potential advantage of the PCR assay is its sensitivity with a detection limit of 102 CFU per ml of bacteria. In all, 79 MRSA isolates recovered from various samples of pigs were subjected to the amplification by the triplex-PCR assay and all the isolates yielded three bands corresponding to the three genes under this study. No false-positive amplification was observed, indicating the high specificity of the developed triplex-PCR assay. This assay will be a useful and powerful method for differentiation of MRSA from methicillin-sensitive S. aureus, coagulase-negative methicillin-resistant staphylococci and coagulase-negative methicillin-sensitive staphylococci. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Letters in Applied Microbiology |
| NAAS Rating: | 8.17 |
| Volume No.: | 72(2) |
| Page Number: | 121-125 |
| Name of the Division/Regional Station: | Animal Science |
| Source, DOI or any other URL: | https://doi.org/10.1111/lam.13408 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/49408 |
| Appears in Collections: | AS-NRCMeat-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.