KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/50414| Title: | Development of SRAP and SSR marker-based genetic linkage maps of guava (Psidium guajava L.) Scientia Horticulturae 192: 158-165 |
| Other Titles: | Not Available |
| Authors: | Padmakar, B; Kanupriya, C; Latha, P Madhavi; Prashant, KS; Dinesh, MR; Sailaja, D; Aswath, C |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Horticultural Research ICAR::Indian Institute of Horticultural Research |
| Published/ Complete Date: | 2015-01-01 |
| Project Code: | Not Available |
| Keywords: | Guava Genotyping M13-tailed PCR Simple sequence repeat Sequence-related amplified polymorphism |
| Publisher: | Not Available |
| Citation: | 13 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Abstract In the present study genotyping of guava full-sib population with 94 F1 progenies, derived from a two-way pseudo-testcross strategy differing in fruit quality traits such as seed strength (hardness/softness), fruit weight, and TSS, was carried out using simple sequence repeat (SSR) markers and sequence-related amplified polymorphic (SRAP) primer combinations. In the case of SSR's high-throughput genotyping, through a M13-Tailed PCR principle, using a set of 160 SSR primer pairs revealed 64.3% parental polymorphism that generated 321 alleles during the mapping population survey. Twenty percent of parental polymorphism was revealed with SRAPs that generated 126 scorable markers. Two linkage maps were constructed for parents using maternal-specific 143 testcross marker loci and paternal-specific 127 testcross markers along with 60 intercross marker loci. At a minimum logarithm of the odds (LOD) score of 4.0 and a maximum map distance of 40 cM the maternal Kamsari map covered 2551.3 cM with an average marker interval distance of 13.21 cM having a total of 193 framework marker loci being ordered into 11 linkage groups. The paternal Purple Local map covered 2113.0 cM with an average marker interval distance of 12.07 cM having 175 framework marker loci being ordered into 11 linkage groups. The estimated genome coverage was of 87.32% in Kamsari and 83.74% in Purple Local. These genetic maps will play a pivotal role in identification of complex quantitative trait loci (QTLs) related to fruit quality. This is the first report of linkage maps in guava based on the combination of SSR along with SRAP markers |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Journal |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Scientia Horticulturae |
| NAAS Rating: | 8.77 |
| Volume No.: | 192: |
| Page Number: | 158-165 |
| Name of the Division/Regional Station: | PGR |
| Source, DOI or any other URL: | https://doi.org/10.1016/j.scienta.2015.04.031 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50414 |
| Appears in Collections: | HS-IIHR-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.