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http://krishi.icar.gov.in/jspui/handle/123456789/50625| Title: | An efficient method of genomic DNA isolation from pomegranate. |
| Other Titles: | Not Available |
| Authors: | Kanupriya, Nischita P. and K.V. Ravishankar |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Horticultural Research |
| Published/ Complete Date: | 2013-01-01 |
| Project Code: | Not Available |
| Keywords: | pomegranate, gDNA, |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Protocol for extracting intact genomic DNA of reasonable quality and purity from minor and underexploited and secondary metabolite rich crops like pomegranate is not available. The presence of large amount of interfering components like polyphenols, polysaccharides, and other secondary metabolites make DNA isolation from pomegranate challenging. The method described involves a modification of the available CTAB method employing silica matrix which yielded high quality DNA. The protocol has been successfully tested with a wild pomegranate genotype and Hind III enzyme digestion. Results show that the DNA isolated is good enough for downstream analysis. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Horticulture |
| NAAS Rating: | 6.16 |
| Volume No.: | 70(4), |
| Page Number: | 584-586 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://www.indianjournals.com/ijor.aspx?target=ijor:ijh&volume=70&issue=4&article=023 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50625 |
| Appears in Collections: | HS-IIHR-Publication |
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