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http://krishi.icar.gov.in/jspui/handle/123456789/50632| Title: | Modifying DNA methylation pattern in papaya embryos to harness useful and stable variants. |
| Other Titles: | Not Available |
| Authors: | Kanupriya Chaturvedi Manamohan, M Leela Sahijram |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Horticultural Research |
| Published/ Complete Date: | 2019-10-01 |
| Project Code: | Not Available |
| Keywords: | Carica papaya, MS-RAPD-PCR, zygotic embryo |
| Publisher: | HSI |
| Citation: | Not Available |
| Abstract/Description: | The purpose of this study was to investigate the effect of various DNA methylation modifying agents on genome wide global DNA methylation status and to develop papaya cv. Surya plants with useful and stable horticulturally important variations. DNA methylation-status modifying agents (Zebularine), 5-azacytidine, 5-aza deoxycytidine (DNA hypomethylating agents), 3’-azido-deoxythymidine (DNA hypermethylating agent) were used at two concentrations (0.3mM and 0.5mM) on papaya cv. Surya zygotic embryos for incubation periods ranging from 0-120 hrs. The technique of MS-RAPD-PCR which resolves global DNA methylation pattern was employed. A total of 87 primers were tested on the control samples, 55 primers showed PCR amplification, of which 11 showed polymorphic bands. Six primers showing clear and reproducible bands were selected further for analysing methylation-modifying-agent treated samples. This technique was found to be reliable and efficient for detecting changes in DNA methylation at both the scale and pattern in a genome wide standpoint. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Horticulture |
| NAAS Rating: | 6.16 |
| Volume No.: | 76 (3) |
| Page Number: | 522-527 |
| Name of the Division/Regional Station: | Fruit Crops |
| Source, DOI or any other URL: | 10.5958/0974-0112.2019.00082.3 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50632 |
| Appears in Collections: | HS-IIHR-Publication |
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