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http://krishi.icar.gov.in/jspui/handle/123456789/50957| Title: | Polymerase chain reaction (PCR) based indexing of black pepper (Piper nigrum L.) plants against Piper yellow mottle virus |
| Other Titles: | Not Available |
| Authors: | A. I. Bhat A. Siljo M.V.Jiby C.K.Thankamani P.A.Mathew |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR-Indian Institute of Spices Research |
| Published/ Complete Date: | 2009-06-01 |
| Project Code: | Not Available |
| Keywords: | black pepper, indexing, PCR, Piper nigrum, Piper yellow mottle virus |
| Publisher: | Indian Society for Spices |
| Citation: | Bhat, A.I., Siljo, A., Jiby, M.V., Thankamani, C.K. and Mathew. P.A. 2009. Polymerase chain reaction (PCR) based indexing of black pepper (Piper nigrum L.) plants against Piper yellow mottle virus. Journal of Spices and Aromatic Crops, 18: 28-32. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A polymerase chain reaction (PCR) based method was used for indexing 845 plants representing 14 popular varieties of black pepper (Piper nigrum) showing no visible external symptoms (apparently healthy) for Piper yellow mottle virus (PYMoV), among which 694 plants were positive (82%) for PYMoV. The percentage of infected plants ranged from 59% to 100% in different varieties; 100% of the tested plants of Panniyur-6 were infected with PYMoV and Sreekara had the least number of infected plants (59%). Some of the indexed PCR positive plants exhibited visible symptoms such as mild chlorosis, yellow specks and mottling in 1-3 months after testing. The study showed that indexing by PCR successfully detected PYMoV in infected black pepper plants showing no visible symptoms. The method can be used in certification programmes to identify PYMoV-free plants for further propagation |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Spices and Aromatic Crops |
| Journal Type: | Research Journal |
| Volume No.: | 18 |
| Page Number: | 28-32 |
| Name of the Division/Regional Station: | Crop Protection |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/50957 |
| Appears in Collections: | HS-IISR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| PYMoV-PCR based detection.pdf | 906.73 kB | Adobe PDF | View/Open |
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