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http://krishi.icar.gov.in/jspui/handle/123456789/51119| Title: | Assessment of genetic relatedness among okra genotypes [abelmoschus esculentus (l.) Moench] using rapd markers |
| Other Titles: | Not Available |
| Authors: | K. Prakash*, M. Pitchaimuthu and K.V. Ravishankar |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | Department of Horticulture, University of Agricultural Sciences, GKVK Campus, Bangalore-50065, India |
| Published/ Complete Date: | 2011-03 |
| Project Code: | Not Available |
| Keywords: | Abelmoschus esculentus (L.) Moench, Genetic diversity, RAPD |
| Publisher: | Indian Society of Plant Breeders |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Abstract: DNA based RAPD (Randomly Amplification of Polymorphic DNA) markers have been used extensively to study genetic relationships in number of crop plants. In this study, 44 okra genotypes collected from different parts of India, were selected to assess genetic distinctiveness and relatedness. Total genomic DNA was extracted and subjected to RAPD analysis using 14 arbitrary 10 mer primers. The molecular analysis showed that all the fourteen primers used revealed clear distinction between the genotypes and they generated a total of 104 RAPD bands most of which were polymorphic across accessions (74.03%). The number of bands resolved per amplification was primer dependent and varied from 4 (OPV-07, OPV-08) to 11 (OPD-05) with average number of bands per primer was 7.41. RAPD data were used to calculate a Squared Euclidean Distance matrix, and based on this, cluster analysis was done using minimum variance algorithm. Cluster analysis showed two major groups. Each sub-group was characterized using morphological and genetic characteristics of the respective genotypes. |
| Description: | Not Available |
| ISSN: | 0975-928X |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Electronic Journal of Plant Breeding |
| Journal Type: | Journal Article |
| NAAS Rating: | 5.14 |
| Volume No.: | 2(1) |
| Page Number: | 80-86 |
| Name of the Division/Regional Station: | Division of Vegetable Crops ICAR-IIHR |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/51119 |
| Appears in Collections: | HS-IIHR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 18.Prakash et.al 2011. Elec.Jl.of Pl. breeding.pdf | 424.36 kB | Adobe PDF | View/Open |
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