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http://krishi.icar.gov.in/jspui/handle/123456789/51392| Title: | Chimeric foot-and-mouth disease virus serotype O displaying a serotype Asia1 antigenic epitope at the surface |
| Authors: | Jitendra K Biswal Rajeev Ranjan Bramhadev Pattnaik |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Project Directorate on Foot and Mouth Disease |
| Published/ Complete Date: | 2016-09-01 |
| Keywords: | Asia1 epitope; Chimeric foot-and-mouth disease virus; Epitope insertion; Foot-and-mouth disease virus; Reverse genetics |
| Publisher: | Springer |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Objective: To determine whether the G-H loop of foot-and-mouth disease virus (FMDV) serotype O can function as a target structure to harbour and display serotype Asia1 antigenic epitope at the surface. Results: Using reverse genetics, FMDV serotype O IND R2/1975 displaying a FMDV serotype Asia1 B cell epitope at the capsid surface was constructed. The epitope-inserted recombinant chimeric virus was genetically stable up to ten serial passages in cell culture and exhibited growth properties similar to the parental serotype O virus. Furthermore, the surface-displayed Asia1 epitope able to react with serotype Asia1 specific antibodies in a competitive ELISA. Importantly, the recombinant chimeric virus showed neutralizing activity to both serotype O and Asia1 polyclonal antibodies. Conclusion: The capsid protein of FMDV serotype O can effectively display potent epitope of other serotypes, making this an attractive approach for the design of new generation bi-valent FMD vaccines. |
| ISSN: | Not Available |
| Type(s) of content: | Journal |
| Language: | English |
| Name of Journal: | Biotechnology Letter |
| Journal Type: | Research |
| NAAS Rating: | 7.98 |
| Volume No.: | 38(9) |
| Page Number: | 1509-17 |
| Source, DOI or any other URL: | 10.1007/s10529-016-2121-4 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/51392 |
| Appears in Collections: | AS-PDFMD-Publication |
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