Detection of A1 and A2 genetic variants of beta-casein in Indian crossbred cattle by PCR-ACRS.

Abstract

Beta-casein A1 variant of milk protein has been found to be a source of β-casomorphins and is correlated with the eti ology of various human disorders like diabetes, ischemic heart disease and sudden infant death syndrome. Therefore, from the applied perception, it is a prerequisite to screen animals for A1 and A2 like genetic variants of β-casein. DNA fragments containing A1/A2 polymorphic region were amplified and genotyped using the PCR-ACRS (Amplification Created Restriction Site). Indian crossbred, Karan Fries, showed an allelic frequency of A1- 0.208 and A2- 0.792 with genotypic frequency of A1A1-0.125, A1A2- 0.166 and A2A2- 0.709. The allelic frequency of A1 and A2 in Karan Swiss was found to be 0.107 and 0.893, respectively, with genotypic frequency of A1A1- 0.0, A1A2- 0.214 and A2A2- 0.786. These two cattle population were developed under a crossbreeding program with exotic breeds (Holstein Friesian and Brown Swiss) which may serve as carriers of the A1 allele because the indigenous cattle breeds (Sahiwal and Tharparkar) have no A1 variant