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http://krishi.icar.gov.in/jspui/handle/123456789/51825| Title: | Engineering foot-and-mouth disease virus serotype O IND R2/1975 for one-step purification by immobilized metal affinity chromatography |
| Authors: | Jitendra K Biswal Punam Bisht Saravanan Subramaniam Rajeev Ranjan Gaurav K Sharma Bramhadev Pattnaik |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Project Directorate on Foot and Mouth Disease |
| Published/ Complete Date: | 2015-09-01 |
| Keywords: | Affinity chromatography; FMD virus; FMDV purification; His(6)-tagged FMDV; Reverse genetics. |
| Publisher: | Elsevier |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Immobilized metal affinity chromatography (IMAC) allows for the efficient protein purification via metal affinity tag such as hexa-histidine (His6) sequence. To develop a new chromatography strategy for the purification and concentration of foot-and-mouth disease virus (FMDV) particles, we inserted the His6-tag at the earlier reported site in the VP1 G-H loop of the FMD virus serotype O vaccine strain IND R2/1975. Display of the His6-tag on the capsid surface, endowed the virus with an increased affinity for immobilized nickel ions. We demonstrated that the His6-tagged FMDV could be produced to high titre and purified from the infected BHK-21 cell lysates by IMAC efficiently. Further, a 1150-fold reduction in protein contaminant level and an 8400-fold reduction in DNA contaminant level were achieved in the IMAC purification of His6-tagged FMDV. Through various functional assays it has been found that the tagged virus retains its functionality and infectivity similar to the non-tagged virus. The affinity purification of the His6-tagged FMDV may offer a feasible, alternative approach to the current methods of FMDV antigen purification, concentration and process scalability. |
| ISSN: | Not Available |
| Type(s) of content: | Journal |
| Language: | English |
| Name of Journal: | Biologicals |
| Journal Type: | Research |
| NAAS Rating: | 7.8 |
| Volume No.: | 43(5) |
| Page Number: | 390-8 |
| Source, DOI or any other URL: | 10.1016/j.biologicals.2015.06.001 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/51825 |
| Appears in Collections: | AS-PDFMD-Publication |
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