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http://krishi.icar.gov.in/jspui/handle/123456789/53133| Title: | Effect of breeding season on in vivo oocyte recovery and embryo production in non-descriptive Indian River buffaloes (Bubalus bubalis). |
| Other Titles: | Not Available |
| Authors: | Manjunatha BM Ravindra JP Gupta PSP Devaraj M Nandi S |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Institute of Animal Nutrition and Physiology |
| Published/ Complete Date: | 2009-01-01 |
| Project Code: | Not Available |
| Keywords: | OPU Buffalo Oocyte Embryo Season |
| Publisher: | Elsevier Ltd |
| Citation: | Manjunatha BM, Ravindra JP, Gupta PSP, Devaraj M and Nandi S (2009) Effect of breeding season on in vivo oocyte recovery and embryo production in non-descriptive Indian River buffaloes (Bubalus bubalis). Animal Reproduction Science. 111:376-383. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The present study was carried out to examine the effect of season on in vivo oocyte recovery and embryo production in non-descriptive, Indian river buffaloes (Bubalus bubalis). Ovum pick up (OPU) was conducted twice a week for 8 weeks during peak (October–March) and low (April–September) breeding season in live buffaloes (n = 6). OPU was performed using ultrasound equipment with a 5 MHz transvaginal transducer, a single lumen 18-gauge, 55-cm long needle and a constant vacuum pressure of 110 mmHg. The number and size of follicles was determined before puncture. The recovered oocytes were graded and only grade A and grade B oocytes were used for in vitro production (IVP) of embryos. The mean number of follicles observed per animal per session did not differed (P < 0.05) between animals or between puncture sessions in both low and peak breeding seasons. Higher (P < 0.05) number of follicles were observed (4.8 ± 0.2 versus 3.1 ± 0.3) and punctured (4.0 ± 0.2 versus 2.4 ± 0.2) during peak breeding season when compared to low breeding season. Oocytes recovered (1.6 ± 0.1 versus 1.0 ± 0.3) per animal per session were higher (P < 0.05) in peak breeding season than low breeding season. During the peak breeding season, the blastocyst yield per animal per session (0.3 ± 0.4 versus 0.18 ± 0.4) was higher (P < 0.05) than the low breeding season. However, season did not significantly affect the percentage of oocytes suitable for IVP (grade A + B) and blastocyst production rate. In conclusion, the efficiency of OPU combined with IVP was higher during the peak breeding season than the low breeding season in buffaloes. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Journal |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Animal Production Science |
| NAAS Rating: | 7.22 |
| Volume No.: | 111 |
| Page Number: | 376-383 |
| Name of the Division/Regional Station: | Animal Physiology |
| Source, DOI or any other URL: | https://doi.org/10.1016/j.anireprosci.2008.03.003 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/53133 |
| Appears in Collections: | AS-NIANP-Publication |
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