Modulation of ERF and ethylene biosynthetic genes during fruit development and jelly seed in mango

Abstract

Dashehari cultivar of mango is one of the leading mango varieties of North India and area under this variety is increasing in other parts of the country also. During fruit ripening, jelly seed formation has been experienced as one of the major disorders where Dashehari is commercially grown. However molecular mechanism behind this disorder is uncertain. To decipher role of ethylene forming enzyme isoform and its associated transcription factors in this disorder, we have mined 17 CDS of AP2/ERF genes in transcriptome data of mango jelly seed tissue. Expression analysis (RT-PCR) of four different ACS (ACS1, 2, 10, 12) and two different ACO (ACO1, ACO4), ERF21, ERF39 and IAA/AUX genes (AUX1, 2) was also analyzed during fruit development and ripening stages i.e. 0 dpa, 10 dpa, 20 dpa, unripe pulp, ripe pulp and jelly seed. Expression of ERF21and ERF39 was found to be up-regulated during fruit development, ripening and jelly seed pulp as compared to 0 dpa. ERF21 and ERF39 plays important role during normal fruit development as well as mango ripening and jelly seed formation. ACS2 plays very important role during mango fruit development only while ACS10 and ACS12 are involved in development and ripening. AUX1 plays very important role during mango fruit development and ripening, however, AUX2 does not play any role in normal fruit development and ripening.