KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/55135
Title: | Amplification, cloning and in silico prediction of full length elicitin gene from Phytophthora capsici, the causal agent of foot rot disease of black pepper |
Other Titles: | Not Available |
Authors: | Vijesh Kumar I P, Reena N, Anandaraj M, Eapen S J, Johnson G K and Vinitha K B |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Institute of Spices Research |
Published/ Complete Date: | 2013-06-24 |
Project Code: | Not Available |
Keywords: | Elicitin; P. capsici; Cloning; In silico |
Publisher: | OMICS International |
Citation: | Vijesh Kumar IP, Reena N, Anandaraj M, Eapen SJ, Johnson GK, et al. (2013) Amplification, Cloning and In silico Prediction of Full Length Elicitin Gene from Phytophthora capsici, the Causal Agent of Foot Rot Disease of Black Pepper. J Plant Pathol Microb 4: 181 doi:10.4172/2157-7471.1000181 |
Series/Report no.: | Not Available; |
Abstract/Description: | Elicitins are a family of small proteins secreted by Phytophthora, which induce leaf necrosis in infected plants. Here, we report the cloning of Elicitin gene from P. capsici, an Oomycete plant pathogen which causes significant damage to a broad range of host plants. Elicitin sequence was amplified using primers designed from the known Elicitin genes of other Phytophthora organisms based on their conserved motifs. The PCR amplified product size of 256 bp length and the BLAST analysis of the sequenced product showed perfect match with alpha-elicitin sequences of P. capsici. Subsequently, attempt was made to characterize the complete gene of elicitin from genome sequence information of P. capsici, by querying the amplified product against the genome. Local BLAST search against full genome identified entire coding sequence. Further sequence analysis identified promoter sequence, transcription start site, a leader signal sequence and a core elicitin domain, with a conserved 6 Cysteine residues. In addition, the three dimensional structure of capsicein was modelled, and the binding affinity of sterol and capsicein was studied using molecular docking. The developed model predicted strong binding affinity for Tyr 47. |
Description: | Not Available |
ISSN: | ISSN: 2157-7471 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of Plant Pathology |
NAAS Rating: | 7.15 |
Volume No.: | 4(6) |
Page Number: | 181 |
Name of the Division/Regional Station: | Division of Crop Protection |
Source, DOI or any other URL: | doi:10.4172/2157-7471.1000181 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/55135 |
Appears in Collections: | HS-IISR-Publication |
Files in This Item:
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.