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http://krishi.icar.gov.in/jspui/handle/123456789/56245| Title: | Micropropagation of Mangifera indica L. cv. Kurukkan through somatic embryogenesis |
| Other Titles: | Not Available |
| Authors: | Mishra, M. Shree, Y., Pati, R., Seal,S., Shukla,N.,Kamle,M., Chandra, R. and Srivastava,A. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR-CISH |
| Published/ Complete Date: | 2010-01-01 |
| Project Code: | Not Available |
| Keywords: | Abiotic stress, Kurakkan, Mangifera indica, Polyembryonic, Rootstock, Salt tolerant, Somatic embryogenesis |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Nucellar embryogenesis was induced in Mangifera indica L. cv. Kurakkan, a polyembryonic salt tolerant, dwarfing rootstock. Nucellus tissue excised from 3.5 cm long fruits developed pro-embryonic callus in 19 days of inoculation on modified MS medium supplemented with 4.52µM 2,4- D, 0.05% malt extract and 13.78µM spermidine. Somatic embryogenesis exhibited high frequency (158.33 embryos). However, all the differentiated embryos proliferated on medium having low level of sucrose (4% w/v) and auxin (2.26µM 2,4-D). Most of the proembryonic calli converted into heart shaped and cotyledonary embryos by reducing temperature to 15 o C. Somatic embryos were matured on modified MS medium fortified with 0.38µM ABA, 0.57µM IAA and 30.30µM PEG. Matured somatic embryos germinated (around 30%) on MS medium supplemented with 2.68µM NAA, 11.60µM kinetin and 2736.9µM glutamine. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Genetics and Plant Breeding |
| NAAS Rating: | 6.55 |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | 70(1):1-6 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/56245 |
| Appears in Collections: | HS-CISH-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Micropropagation of Mangifera indica.pdf | 138.04 kB | Adobe PDF | View/Open |
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