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http://krishi.icar.gov.in/jspui/handle/123456789/58793| Title: | In Silico mining of single nucleotide polymorphisms in expressed sequence tags dataset of Allium cepa L |
| Other Titles: | Not Available |
| Authors: | V Radhika DCL Reddy A Bhardwaj C Aswath |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Horticultural Research |
| Published/ Complete Date: | 2011-01-01 |
| Project Code: | Not Available |
| Keywords: | Cap3, contig, SNPDigger, transition, transversion |
| Publisher: | Not Available |
| Citation: | V Radhika, DCL Reddy, A Bhardwaj, C Aswath (2011) In Silico mining of single nucleotide polymorphisms in expressed sequence tags dataset of Allium cepa L. Online Journal of Bioinformatics 12 (1), 34-45 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Single nucleotide polymorphisms (SNPs) are abundant in plant genomes and are thus used frequently in genetic analysis and crop breeding programs. Potential SNPs from expressed sequence tags (ESTs) of Allium cepa were mined for a total of 232 potential SNPs identified from 20,157 ESTs from NCBI. The SNPs occurred on an average frequency of 1 per 57 bp in the EST sequences. The ratio of transitions to transversions was 1.7: 1, and 65 of the 232 SNP sites were cut by different restriction enzymes. Primers could be designed for 227 SNPs and used for onion breeding programs after validation in wet lab. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Online Journal of Bioinformatics |
| Volume No.: | 12 |
| Page Number: | 34-45 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | http://onljbioinform.com/aliumcepaabs2011.htm |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/58793 |
| Appears in Collections: | HS-IIHR-Publication |
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