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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/60740
Title: | Production of Polyclonal Antibody for Oxytetracycline and their use in Lateral Flow Assay. |
Other Titles: | Not Available |
Authors: | Naik, L. Lata, K.; Sharma, R.; Mann, B. and Rajput, Y.S. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Dairy Research Institute |
Published/ Complete Date: | 2014-12-28 |
Project Code: | Not Available |
Keywords: | Oxytetracycline, Milk, Antibiotic residue, Lateral flow assay. |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | A new synthesis way of haptens for oxytetracycline (OTC) with Keyhole limpet hemocyanin (KLH) was demonstrated. This hapten conjugate (OTC-KLH) were used to successfully raise polyclonal antibodies against OTC. Utilizing this antibodies a rapid, user friendly and sensitive lateral flow assay (LFA) was designed to detect the OTC in milk system. From this assay results could be accomplished within 5 min. without the need of any equipment. The visual detection limit was 30 μg/kg. Proposed concept of OTC antibody production found to be a simple and reliable method for LFA. Thus, the developed assay could be used in the milk system to carry out onsite screening for OTC, from the beginning of the food chain to improve commercial trade and to uphold the consumer safety. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Not Available |
Volume No.: | Not Available |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/60740 |
Appears in Collections: | AS-NDRI-Publication |
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