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http://krishi.icar.gov.in/jspui/handle/123456789/60753| Title: | Lateral flow assay based rapid detection of cephalexin in milk. |
| Other Titles: | Not Available |
| Authors: | Lata, K.; Sharma, R.; Naik, L.; Rajput, Y.S. and Mann, B. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Dairy Research Institute |
| Published/ Complete Date: | 2015-06-28 |
| Project Code: | Not Available |
| Keywords: | CEPHALEXIN, MILK |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A rapid and semiquantitative lateral flow assay has been developed for detection of cephalexin (CFX) in milk. The assay is based on a competitive format using polyclonal antibodies. Antibodies were raised in rabbits against CFX-keyhole limpet hemocyanin. The specificity of antibodies was ascertained by direct enzyme-linked immunosorbent assay. The laboratory prepared gold nanoparticles coupled to anti-CFX antibodies, which competed for free CFX in sample and CFX–bovine serum albumin conjugate on the test line of a lateral flow strip. The control line of the lateral flow strip consisted of species-specific antibody. The assay was validated with spiked milk samples and involved application of skim milk to the adsorbent pad followed by visualization of red color on test and control lines within 8 min. The lower detection limit of CFX was 30 ppb in milk, which is lower than the Codex prescribed maximum residue limit. The storage stability of the prepared strip was also established |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Food Quality |
| NAAS Rating: | 7.76 |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/60753 |
| Appears in Collections: | AS-NDRI-Publication |
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