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http://krishi.icar.gov.in/jspui/handle/123456789/60754| Title: | Rapid enzymatic assay for lactate content in milk – an indirect method to ascertain the presence of neutralizers in microbial deteriorated milk. |
| Other Titles: | Not Available |
| Authors: | Kumar, B.; Sharma, R.; Thakur, R.; Barui, A.K.; Rajput, Y.S. and Mann, B. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Dairy Research Institute |
| Published/ Complete Date: | 2016-02-16 |
| Project Code: | Not Available |
| Keywords: | lactate, lactate oxidase |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A rapid method for qualitative and quantitative assaying of l-lactate in milk is described. The method is based on oxidation of lactate by lactate oxidase with the simultaneous formation of H2O2 which subsequently oxidises 2, 4, 6-tribromo 3-hydroxy benzoic acid (TBHBA) in the presence of peroxidase. Oxidised TBHBA then reacts with 4-amino antipyrine to obtain magenta colour (λmax = 523 nm). In the qualitative assay, colour change becomes visible in raw milk samples spiked with 300 mg lactate/L. The absorbance increases linearly up to 1000 mg/L. The method can be used for rapid assessment of the hygienic quality of milk. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Not Available |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/60754 |
| Appears in Collections: | AS-NDRI-Publication |
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