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http://krishi.icar.gov.in/jspui/handle/123456789/60761| Title: | Development of competitive indirect enzyme-linked immunosorbent assay for detection of cephalexin in milk. |
| Other Titles: | Not Available |
| Authors: | Lata, K.; Naik, L.; Sharma, R.; Jaiswal, A.; Mann, B. and Rajput, Y.S. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Dairy Research Institute |
| Published/ Complete Date: | 2017-01-01 |
| Project Code: | Not Available |
| Keywords: | Lata, K.; Naik, L.; Sharma, R.; Jaiswal, A.; Mann, B. and Rajput, Y.S. |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | In this study, a rapid competitive enzyme-linked immunosorbent assay (ELISA) was developed for the detection of cephalexin (CFX) residues in milk. A simple strategy for the production of haptens for CFX was demonstrated with keyhole limpet hemocyanin. This hapten conjugate was used as immunogen to successfully raise the polyclonal antibodies against CFX. Utilizing these antibodies, an ELISA was developed to screen CFX residues in milk. The detection limit was calculated as 10 ppb in milk, which was lower than the European Union prescribed Maximum Residue Limit of 100 ppb in milk. Recovery of CFX from spiked milk sample was quantitative. The method can be used for rapid screening of milk samples for CFX residues. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Not Available |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/60761 |
| Appears in Collections: | AS-NDRI-Publication |
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