Nano zinc supplementation in goat (Capra hircus) ration improves immunity, serum zinc profile and IGF-1 hormones without affecting thyroid hormones.

Abstract

The trial was aimed at evaluating probable superiority, if any of nano zinc (NZn) over inorganic zinc (Zn) on immunity, serum minerals and T3 , T4 , and IGF-1 hormone profiles in goats. NZn was synthesized by using 0.45 M aqueous solution of Zn nitrate and 0.9 M aqueous solution of sodium hydroxide (average particle size 74 nm). Twenty-four male goats were grouped into four groups as per their body weight and were supplemented with either a basal diet with concentrate and straw at 50:50 ratio (Negative control, NC) alone or supplemented with 50 mg/kg Zn (Control) from inorganic Zn source, that is ZnO (IZn-50), 50 mg/kg Zn from NZn (NZn-50) or 25 mg/kg Zn from NZn (NZn-25). No change was observed in thyroid hormone status on zero and 90th day of experimental feeding, but NZn supplementation improved (p < 0.05) IGF-1 level on 90th day serum samples. Zn supplementation improved the humoral immunity in all the groups irrespective of the source. Similarly, cell-mediated immunity (CMI) measured by skinfold thickness after injecting Con-A, was also improved in Zn supplemented groups than control at 6, 12 and 48 h of incubation. NZn-50 animals showed highest HI (haemagglutination inhibition) titre as well as skin thickness. The CD+4 (cluster of differentiation in %) was more (p < 0.05) in Zn supplemented groups. NZn-50 showed higher (p < 0.05) CD+8 count than NC and similar (p > 0.05) to IZn-50 and NZn-25 groups without affecting (p > 0.05) the ratio of CD+4,CD+8 among the treatment groups. Thus, NZn supplementation at 25 mg/kg had similar immunity and serum T3 , T4 and IGF-1 profiles compared with IZn at 50 mg/kg dose