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http://krishi.icar.gov.in/jspui/handle/123456789/7187| Title: | Expression and purification of recombinant H5HA1 protein of H5N1 avian influenza virus in E. coli and its application in indirect ELISA |
| Other Titles: | Not Available |
| Authors: | Redda Y.T. Venkatesh G. Kalaiyarasu S. Bhatia S. Kumar D.S. Nagarajan, S. Pillai A. Tripathi S. Kulkarni D.D. Dubey S.C. |
| Author's Affiliated institute: | ICAR::National Institute of High Security Animal Diseases |
| Published/ Complete Date: | 2016-02-01 |
| Project Code: | Not Available |
| Keywords: | AIV H5N1 Characterization Expression Haemagglutinin Indirect ELISA |
| Publisher: | Marcel Dekker Inc. |
| Citation: | Redda YT, Venkatesh G, Kalaiyarasu S, Bhatia S, Kumar DS, Nagarajan S, Pillai A, Tripathi S, Kulkarni DD, Dubey SC (2016). Expression and purification of recombinant H5HA1 protein of H5N1 avian influenza virus in E. coli and its application in indirect ELISA. J Immunoassay Immunochem. J Immunoassay Immunochem. 2016;37(4):346-58. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The PCR amplified HA1 fragment of H5N1 (H5HA1) avian influenza virus (AIV) hemagglutinin gene was cloned into pET28a ( + ) expression vector and expressed in Rosetta Blue (DE3) pLysS cells. The recombinant H5HA1 (rH5HA1) protein purified by passive gel elution after SDS-PAGE of the inclusion bodies reacted specifically with H5N1 serum in Western blot analysis. A subtype specific indirect enzyme linked immunosorbent assay (iELISA) using the rH5HA1 protein as the coating antigen was developed for detecting antibodies to H5 subtype of AIV. The assay had 89.04% sensitivity and 95.95% specificity when compared with haemagglutination inhibition test. The Kappa value of 0.842 indicated a perfect agreement between the tests. The iELISA developed can be used for serosurveillance of avian influenza in chickens. |
| Description: | Not Available |
| ISSN: | 15324230 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Immunoassay and Immunochemistry |
| Volume No.: | 37(4) |
| Page Number: | 346-358 |
| Name of the Division/Regional Station: | Biochemistry Lab |
| Source, DOI or any other URL: | 10.1080/15321819.2015.1135160. |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/7187 |
| Appears in Collections: | AS-NIHSAD-Publication |
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