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http://krishi.icar.gov.in/jspui/handle/123456789/7308| Title: | Species identification of cattle and buffalo fat through PCR assay |
| Other Titles: | Not Available |
| Authors: | S. Vaithiyanathan and V. V. Kulkarni |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Research Centre on Meat, Hyderabad |
| Published/ Complete Date: | 2016-12-31 |
| Project Code: | Not Available |
| Keywords: | Cattle Buffalo Fat mt DNA PCR |
| Publisher: | Springer Link |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A method was standardized to isolate quality DNA from cattle and buffalo fat for species identification using QIAamp DNA stool mini kit. The quality of the DNA was sufficient enough to amplify universal primers viz., mt 12S rRNA and mt 16S rRNA, and species specific D loop primers for cattle and buffalo. The sensitivity of the PCR assay in the species specific D loop primer amplification was with a detection level of 0. 47 ng cattle DNA and 0.23 ng buffalo DNA in simplex and, 0. 47 ng cattle DNA and 0.12 ng buffalo DNA in duplex PCR. It is a potentially reliable method for DNA detection to authenticate animal fat. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Food Science and Technology |
| NAAS Rating: | 7.95 |
| Volume No.: | 53(4) |
| Page Number: | 2077-2082 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.1007/s13197-016-2198-8 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/7308 |
| Appears in Collections: | AS-NRCMeat-Publication |
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